Figure 6. Myogenic, adipogenic and osteogenic differentiation of USPIO-labeled ADSCs after 3 weeks induction.
Red-colored lipid vacuoles were detected by Oil Red staining. Calcium deposits were found in osteogenically differentiated ADSCs. Myoblast differentiation exhibited a significant upregulation of α-SMA expression. (A) α-SMA expression in USPIO-labeled ADSCs. (B) Oil red staining of USPIO-labeled ADSCs. (C) Alizarin red staining of USPIO-labeled ADSCs. (D) α-SMA expression in unlabeled ADSCs. (E) Oil red staining of unlabeled ADSCs. (F) Alizarin red staining of unlabeled ADSCs. Scale bars: 100 μm.