Figure 1. Schematic overview of carbohydrate array construction and use.

After formation of a hydrophobic SAM of alkanethiols on a gold-coated target, hexadecylamine-labelled oligosaccharides are immobilised via hydrophobic-interaction. Products of enzyme activity, here exemplified by removal of DP2, as well as the original substrate, can be identified with MALDI-ToF MS.