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. 2017 Feb 21;7:43117. doi: 10.1038/srep43117

Figure 4. MALDI-ToF MS analysis of commercial enzyme activity on carbohydrate arrays.

Figure 4

Spectra showing reaction products of incubation of (A) β-glucosidase on cellotetraose-containing arrays, with DP4 + Na 914.5 m/z, DP4 + H 892.5 m/z, DP3 + Na 752.4 m/z, DP3 + H 730.5 m/z, (B) endoxylanase on xylohexaose-containing arrays with DP6 + H 1036.7 m/z, DP5 + H 904.6 m/z, DP4 + H 772.6 m/z, DP3 + H 640.6 m/z. Reaction conditions were chosen such that incomplete substrate degradation allowed identification of both substrates and products in one mass spectrum. Intensities of peak resulting from background substrate degradation or impurities were ≤2.2% (Fig. S3).