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. 2017 Feb 21;7:42926. doi: 10.1038/srep42926

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Schematic diagram of a coronal mouse brain section indicating the region removed using biopsy punch pen. Modified from Allen Brain Atlas (http://atlas.brain-map.org/#atlas=1&plate=100960236&structure=549&x=5280&y=3744.0000697544647&zoom=3&resolution=11.97&z=5). (b) Flow chart to illustrate fractionation methodology. TxP = Triton X-100 insoluble synaptic fraction (pellet of last spin), TxS = Trinton X-100 soluble extrasynaptic membrane fraction (supernatant of last spin). (c) Representative WB composite images showing bands corresponding to markers, Pan-Cadherin and PSD-95 in different fractions. S1 = supernatant 1 - total lysate; S2 = supernatant 2 - cytosol; TxS = Triton X-100 soluble supernatant – extrasynaptic membrane; TxP = Triton X-100 insoluble pellet – synaptic membrane.