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. 2017 Feb 21;7:43096. doi: 10.1038/srep43096

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Copyright © 2017, The Author(s)

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(a) Detection of NO by staining with fluorescent dye DAF-FM DA at 24 h after OTC treatment. The fluorescence intensity for NO accumulation in the root tips is shown in each image. Data are the means of three replicates with SE (*P value < 0.05, two-sided Student’s t test). (b,c) Effects of 250 μM cPTIO (the NO scavenger), and 100 μM tungstate (the inhibitor of nitrate reductase, NR) on root growth of tomato seedlings in the presence or absence of 10 μM OTC. Measurements were taken 3 d after treatments, and data represent mean ± SE (n = 15). Means followed by the same letter are not significantly different according to Tukey’s test (P < 0.05). (d) Effect of treatment with OTC on expression of NR gene in the root tips. Samples were collected 24 h after treatment. Data represent mean ± SE (n = 5, *P value < 0.05, two-sided Student’s t test).