Table 2.
HCV PCR test results (log10 IU/ml) of original samples (n = 43) in comparison to the test results after storage for 6–8 days at 4 ° C (2–8 ° C) and the retested samples after storage at ≤–18 ° C
| No. | A original sample | B* storage at ≤–18 °C | Viral load reduction B – A | E* storage at 4 °C, 6–8 days | Viral load reduction E – A | Viral load reduction E – B |
|---|---|---|---|---|---|---|
| 1 | 2.81 | n.d. | − | 2.13 | −0.68 | − |
| 2 | 3.22 | 3.07 | −0.15 | 3.08 | −0.14 | 0.01 |
| 3 | 3.57 | n.d. | − | 3.14 | −0.43 | |
| 4 | 3.76 | 3.98 | 0.22 | 3.63 | −0.13 | −0.34 |
| 5 | 3.77 | 3.87 | 0.10 | 3.35 | −0.43 | −0.52 |
| 6 | 4.10 | n.d. | − | 3.76 | −0.33 | − |
| 7 | 4.11 | n.d. | − | 3.58 | −0.54 | − |
| 8 | 4.12 | 3.74 | −0.38 | n.d. | − | − |
| 9 | 4.13 | n.d. | − | 4.02 | −0.11 | − |
| 10 | 4.14 | n.d. | − | 3.44 | −0.70 | − |
| 11 | 4.26 | 3.80 | −0.45 | 3.51 | −0.74 | −0.29 |
| 12 | 4.33 | n.d. | − | 3.23 | −1.11 | − |
| 13 | 4.40 | n.d. | − | 2.74 | −1.66 | − |
| 14 | 4.42 | 3.93 | −0.49 | 2.94 | −1.48 | −0.99 |
| 15 | 4.46 | 4.32 | −0.14 | 3.23 | −1.22 | −1.08 |
| 16 | 4.49 | 4.42 | −0.07 | 3.91 | −0.57 | −0.50 |
| 17 | 4.60 | n.d. | − | 4.73 | 0.13 | − |
| 18 | 4.65 | 4.61 | −0.03 | 4.68 | 0.03 | 0.07 |
| 19 | 4.72 | 4.85 | 0.13 | 4.89 | 0.17 | 0.04 |
| 20 | 4.74 | 5.03 | 0.29 | 4.66 | −0.08 | −0.37 |
| 21 | 4.84 | n.d. | − | 4.30 | −0.54 | − |
| 22 | 4.86 | 5.18 | 0.32 | 4.46 | −0.39 | −0.71 |
| 23 | 4.87 | 4.63 | −0.24 | 4.38 | −0.49 | −0.25 |
| 24 | 4.91 | n.d. | − | 3.65 | −1.26 | − |
| 25 | 4.93 | n.d. | − | 4.47 | −0.46 | − |
| 26 | 4.94 | 4.62 | −0.32 | 3.84 | −1.10 | −0.78 |
| 27 | 4.95 | n.d. | − | n.d. | − | − |
| 28 | 4.97 | 5.05 | 0.08 | 4.90 | −0.07 | −0.15 |
| 29 | 5.06 | 4.89 | −0.18 | 4.91 | −0.16 | 0.02 |
| 30 | 5.24 | 5.14 | −0.10 | 5.25 | 0.01 | 0.11 |
| 31 | 5.30 | 5.23 | −0.07 | 5.23 | −0.07 | 0.00 |
| 32 | 5.36 | 5.49 | 0.14 | 5.20 | −0.16 | −0.30 |
| 33 | 5.36 | 4.89 | −0.47 | 4.36 | −1,00 | −0.53 |
| 34 | 5.38 | 5.41 | 0.03 | 5.22 | −0.16 | −0.19 |
| 35 | 5.66 | 5.17 | −0.49 | 5.43 | −0.23 | 0.26 |
| 36 | 5.69 | 5.43 | −0.25 | 5.32 | −0.37 | −0.12 |
| 37 | 5.76 | 5.69 | −0.07 | 5.84 | 0.08 | 0.15 |
| 38 | 5.87 | 5.92 | 0.05 | 5.94 | 0.07 | 0.03 |
| 39 | 5.90 | 5.90 | 0.00 | 5.97 | 0.08 | 0.07 |
| 40 | 6.00 | 6.13 | 0.13 | 6.06 | 0.06 | −0.07 |
| 41 | 6.37 | 6.66 | 0.30 | 5.13 | −1.23 | −1.53 |
| 42 | 6.49 | 6.62 | 0.13 | 6.61 | 0.13 | 0.00 |
| 43 | 6.81 | 7.15 | 0.34 | 5.28 | −1.53 | −1.87 |
| Mean | −0.06 | −0.46 | −0.34 | |||
| Maximal viral load reduction | −0.49 | −1.66 | −1.87 | |||
n.d. = Not done; – = not calculable, since no sufficient sample volume was available for testing. However, these samples were tested at other times points (e.g. 1–3 days and/or 3–5 days).
*
Test values were multiplied by the dilution factor