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. 2017 Feb 21;7:42990. doi: 10.1038/srep42990

Figure 5. Effect of TWIST1 on TET1 expression in TC28 cells.

Figure 5

(A) Schematic representation of the human TWIST1 protein. (B) Myc-tagged TWIST1 or TWIST1-ΔbHLH stably transfected TC28 cells were generated. Myc expression was analyzed by Western blotting. (C) TET1, 2, and 3 gene expression levels in TWIST1 stably transfected TC28 cells (TC28-TWIST1) were determined by quantitative PCR. Stable TWIST1-ΔHLH transfected TC28 cells (TC28-TWIST1-ΔHLH) were used as control. (D) Western blot analysis of TET 1, 2 and 3 expression in TC28-TWIST1 and TC28-TWIST1-ΔHLH cells. All gels for western blot analysis were run under the same experimental conditions, and western blot images were cropped with a grey cropping line. (E) Immunohistochemistry analysis of human cartilage tissues. Cartilage sections were obtained from normal and OA-affected cartilages. Sections were stained with TET1 anti body. Scale bar, 200 μm.