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. 2017 Feb 21;7:42990. doi: 10.1038/srep42990

Figure 6. MMP3 expression change after TWIST1 overexpression under TET knock down.

Figure 6

(A) The siRNA for TET1 was transfected to human primary chondrocytes, and after 36 hours, cells were infected with Ad-GFP or Ad-TWIST1 and cultured for 72 hours. The MMP3 gene expression was determined by real-time qRT-PCR. (B) Fibroblasts were derived from Tet1, 2 and 3 triple knock out mice embryonic stem cells, and were transfected Ad-GFP or Ad-TWIST1 for 72 hours. Mmp3 gene expression was determined by quantitative PCR. *P < 0.05.