Table 2.
Specimens of laboratory colony Aedes japonicus PEN (n = 10) examined microscopically for larval stages of Dirofilaria immitis
| Mosquitoes | Number of larval stages per individual positive mosquito | ||||||
|---|---|---|---|---|---|---|---|
| Dpi | Total no. | No. positive | mf | L1 | L2 | L3 | Location (total no. in all dissected mosquitoes)b |
| 1 | 2 | 1a | 2 | – | – | – | Midgut (2) |
| 3 | 3 | 2a | – | 1, 3 | – | – | Malpighian tubules (4) |
| 4 | 1 | 0a | – | – | – | – | |
| 5 | 2 | 1a | 1 | 11 | – | – | Malpighian tubules (11, of which 3 melanized) |
| 6 | 1 | 0a | – | – | – | – | |
| 14 | 1 | 0 | – | – | – | – | |
Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
aOne additional mosquito positive in PCR only
bData given only when localisation was clearly assignable after dissection