Table 4.
Specimens of Aedes japonicus (CH) (n = 24) examined microscopically for larval stages of Dirofilaria immitis
| Mosquitoes | Number of larval stages per individual positive mosquito | ||||||
|---|---|---|---|---|---|---|---|
| Dpi | Total no. | No. positive | mf | L1 | L2 | L3 | Location (total no. in all dissected mosquitoes)c |
| 1 | 3 | 2 | 5, 4 | – | – | – | |
| 3 | 6 | 6 | 1, 1, 1, 1 | 5, 4, 2, 1 | – | – | |
| 4 | 3 | 1b | – | 6 | – | – | |
| 7 | 3 | 2 | – | 7 | 5, 3 | 1 | In Malpighian tubules, L1 melanized |
| 10 | 3 | 2 | – | – | – | 1, 6 | In Malpighian tubules (7) |
| 14 | 3a | 3 | – | – | 1 | 1, 3 | Head (1), Malpighian tubules (2) |
| 21 | 3 | 3 | – | – | 1 | 1, 4, 9 | Proboscis (7), head (1), thorax (5) |
Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
aProboscis of one mosquito was lost during the dissection process
bAdditional two positive in PCR only
cData given only when localisation was clearly assignable after dissection