Table 6.
Specimens of laboratory colony Aedes geniculatus IPZ (n = 9) examined microscopically for larval stages of Dirofilaria immitis
| Mosquitoes | Number of larval stages per individual positive mosquito | ||||||
|---|---|---|---|---|---|---|---|
| Dpi | Total no. | No. positive | Mf | L1 | L2 | L3 | Location (total no. in all dissected mosquitoes)c |
| 1 | 2 | 2 | 5, 6 | – | – | – | |
| 4 | 2 | 0a | – | – | – | – | |
| 7 | 2 | 2b | – | 25 | – | – | Malphighian tubules (24), melanized in Malpighian tubules (1) |
| 9 | 0 | 0b | – | – | – | – | |
| 10 | 2 | 1b | – | – | – | 11 | Malpighian tubules (11) |
| 13 | 1 | 1 | – | – | – | 6 | Abdomen (5), Thorax (1) |
Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
aTwo additional positive in PCR only
bOne additional positive in PCR only
cData given only when localisation was clearly assignable after dissection