Table 8.
Specimens of laboratory colony Aedes aegypti IPNC (n = 22) examined microscopically for larval stages of Dirofilaria immitis
| Number of larval stages per individual positive mosquitoc | ||||||
|---|---|---|---|---|---|---|
| Dpi | Total no. of individuals | No. of positive individuals | mf | L1 | L2 | L3 |
| 1 | 9 | 5a | 1, 1, 5, 7, 10 | – | – | – |
| 2 | 1 | 1 | – | 1 | – | – |
| 3 | 4 | 1b | 1 | – | – | – |
| 5 | 2 | 1 | – | 3 | – | – |
| 7 | 2 | 0a | – | – | – | – |
| 9 | 1 | 0b | – | – | – | – |
| 12 | 1 | 0b | – | – | – | – |
| 14 | 1 | 0b | – | – | – | – |
| 16 | 1 | 0b | – | – | – | – |
Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
aTwo additional positive in PCR
bOne additional positive in PCR only
cLocalisation of developmental stages was not clearly assignable after dissection