Fig. 2.

Silencing of RhoBTB1 causes Golgi fragmentation. HeLa cells were transfected with siRNAs targeting RhoBTB1 or RhoBTB2. After 48 h, the cells were fixed and stained for the Golgi marker giantin (green). a Shows representative images of normal and RhoBTB1 depleted cells. Bar = 10 μm. b Silencing of RhoBTB1 caused a marked and significant fragmentation of the Golgi. Data are means ± SEM (n = 3). Data were normalized to mock-transfected HeLa cells. Comparisons are to the lamin siRNA control; *P <0.05, **P <0.01