Fig. 2.

Detection of the interaction between pUL83 and AIM2. a Schematic diagram of a two-hybrid experiment, adapted from the Matchmaker Mammalian Assay Kit. b Plasmids encoding recombinant pUL83 and AIM2 proteins were used together with pG5SEAP to co-transfect HEK293T cells for 72 h. Supernatants were then collected and SEAP levels were detected by chemiluminescence at 405 nm. The experiment was repeated three times. Statistical data were analyzed using the t-test. c Plasmids encoding recombinant pUL83 and AIM2 were used to transfect HEK293T cells for 72 h. Cells were harvested and lysed with protein lysis buffer, and whole cell lysates were immunoblotted using specific antibodies against pUL83 and AIM2, or immunoprecipitated with the anti-AIM2 antibody and then detected using the anti-pUL83 antibody. IgG was used as a negative control. Data from one representative experiment out of three are presented as the mean ± SD. * P < 0.01. WCL: whole cell lysates