Fig. 10.

Gene expression profiles remained normal after H3K27me3 enrichment was reduced in fetal male germ cells. a Average H3K27me3 intensities were measured using flow analysis of FACS sorted E12.5 germ cells cultured for 72 h with a vehicle control (DMSO; green) or 10 μm GSK126 (blue). b–d Expression microarray relative probe signal intensities normalized to Sdha, Canx and Mapk1 for genes expressed in E12.5 germ cells cultured for 72 h with DMSO or 10 μm GSK126: b Hoxb locus. c A selection of male germ cell development genes; Sox2, Pou5f1, Nanog, Dppa2, Dppa4, Tdrd5, Piwil2, Tdrd7, Nanos2, Dnmt3l, Mvh. d PRC2 genes; Ezh1, Ezh2, Eed and Suz12. Values ± SEM; n = 3 for each treatment