Fig. 1.

Early differentiated T cell selection using CCR7. a Representative plot following Isolation of early differentiated T cells using immuno-magnetic beads against CCR7. A1 CCR7⁺ and A2 CCR7⁻ fractions. The frequency of b CCR7⁺, c CD4⁺ and d CD25⁺ CD127⁻ FoxP3⁺ Tregs in each fraction following CCR7 selection as determined by flow cytometry. NSC Non sorted control T cells. Error bars ± SD, statistical tests one way ANOVAs with Bonferroni’s post-test. e and f The phenotype of CD8⁺ and CD4⁺ T cell populations respectively following CCR7 selection; Naïve (CCR7⁺ CD45RA+), Central memory (CCR7⁺ CD45RA⁻), Effector memory (CCR7⁻ CD45RA⁻), Effector (CCR7⁻ CD45RA⁺). Error bars show ± SEM. For each phenotype; significant difference from NSC T cells is shown. * P < 0.05, **P < 0.01, *** P < 0.001