Table 1.

Summary of PI3K subunit gene mutations identified in TET cell lines and primary tumors

Sample ID	Sample Type	WHO Histotype	Mutation	Gene Symbol	PI3K Subunit	Effect	SIFT/PolyPhen	Sanger Seq
MP57	Cell line & Tumor	Thymic Carcinoma	chr19:18273784G>C c.1117G>C p.G373R	PIK3R2	p85β, Regulatory	Missense Mutation	deleterious/probably damaging	Homozygous
IU-TAB1	Cell line	Thymoma Type AB	chr3:178936091G>A c.1633G>A p.E545K	PIK3CA	p110α, Catalytic	Missense Mutation	deleterious/probably damaging	Heterozygous
N-Thy015	Tumor	Thymic Carcinoma	chr7:106508065G>A c.3309G>A p.R20H	PIK3CG	p110γ, Catalytic	Missense Mutation	deleterious/probably damaging	Confirmed
Padua001	Tumor	Thymic Carcinoma	chr3:130427227C>G c.579C>G p.S173T	PIK3R4	p150, Regulatory	Missense Mutation	benign/tolerated	Confirmed
Padua026	Tumor	Thymic Carcinoma	chr3:178936091G>A c.1068G>A p.E545K	PIK3CA	p110α, Catalytic	Missense Mutation	deleterious/probably damaging	Confirmed