Figure 7. Representative IF images following inoculation with fluorescently labeled LL-37.

(A), (B), and (C) represent tissues harvested 30 minutes after inoculation with LL-37 at 10×, 20×, and 40× magnification, respectively. (D), (E), and (F) represent tissues harvested 24 hours after inoculation at 10×, 20×, and 40×, respectively. Nuclei are counterstained with DAPI (blue). Red represents fluorescent LL-37. At 30 minutes, there is coating of the epithelial layer, as well as bright staining within the lamina propria. At 24 hours, there is absence of fluorescent LL-37 coating the epithelium with minimal evidence of fluorescent LL-37 present in the lamina propria. OE (olfactory epithelium); LP (lamina propria).