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Michelis-Menten kinetic constants of MmARD proteins for the substrate desthio-acireductone
| Protein | KM (µM) | kcat (s−1) | kcat/ KM (M−1s−1) |
|---|---|---|---|
| Fe-MmARD | 118.5 | 111.7 | 9.5E5 |
| Co-MmARD | 49.3 | 7.87 | 1.6E5 |
| Ni-MmARD | 287.1 | 17.1 | 6.0E4 |
The kinetic parameters for substrate desthio-acireductone were obtained at a fixed concentration of the second substrate oxygen (0.25 mM calculated on the basis of solubility of oxygen in water at 25 °C).
