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. 2017 Mar;137(3):760–763. doi: 10.1016/j.jid.2016.11.011

Figure 1.

Figure 1

IMQ-induced skin inflammation and psoriasiform dermatitis is impaired in C3-deficient mice. IMQ was applied for 7 days to the ear of BALB/c WT (black square) and BALB/c.C3–/– (red circle) mice. (a) C3 expression in whole skin quantified by quantitative real-time –PCR 24 hours after the last application (n = 4). Naïve indicates untreated mice, mean ± standard error of the mean relative to cyclophilin, unpaired t test. (b) Representative pictures of IMQ-treated ears at day 7. (c) Ear thickness (mean ± standard error of the mean) and clinical scores (mean ± standard deviation) for erythema and scaling. Representative experiment of two, Mann-Whitney test. (d) Number of CD45+ cells, neutrophils (Ly-6G+), monocytes (CD11b+Ly-6C+Ly-6G–), and γδ T cells (CD4–CD3+γδTCR+) at day 7. (e–f) Proportion of IL-17+ γδ T cells from (e) skin and (f) LN after in vitro restimulation. Mean ± standard error of the mean, unpaired t test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. d, day; IMQ, imiquimod; LN, lymph node; M, meter; no., number; WT, wild type.