Fig. 1. 4T1 mammary tumor growth and lung metastasis in rl^{Orl −/−} mice.

(a) Growth of primary tumors in rl^{Orl −/−} (n=10) and rl^{Orl +/+} (n=10) control mice. (b) Wet weight of primary tumors collected 25 days after 4T1 cell injection. (c) Total body weight of animals on the day of 4T1 injections (t = 0) and the day of sacrifice (t = 25 days). (d) Quantification of metastatic burden in the lungs. (e) Representative images of 4T1-derived metastatic colonies from the lungs of rl^{Orl +/+} and rl^{Orl −/−} mice used for quantification in (d). Bar = 5 mm. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance determined using two-tailed, unpaired Student’s t-test.