Skip to main content
. 2016 Dec 27;66(3):741–753. doi: 10.2337/db16-1338

Figure 1.

Figure 1

Grp170 knockdown impairs Akita degradation. A: 293T cells expressing Akita-Myc (1 µg) were transfected with scrambled or Grp170-specific siRNA. Cells were then treated with 100 μg/mL cycloheximide for the indicated time and harvested. The resulting WCE was analyzed by immunoblotting using the indicated antibodies. B: The Akita-Myc band intensity in A and C was quantified using ImageJ (National Institutes of Health). Data represent the mean ± SD of at least three independent experiments. C: As in A, except cells were transfected with Sil1 siRNA.