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. 2016 Nov 29;66(3):763–778. doi: 10.2337/db16-0695

Figure 7.

Figure 7

Edn1 plus Ednra mediates glomerular endothelial mitochondrial dysfunction and progression of DKD. A: Immunofluorescence detecting mtDNA 8-oxoG in prominent CD31-positive glomerular endothelial cells of 3-week diabetic STZ-D2 (top) and STZ-D2 mice cotreated with BQ-123 (0.1 nmol/kg/day) (bottom). B: Urine 8-oxodG/creatinine levels of control mice, STZ-D2, or STZ-D2 cotreated with BQ-123. ACR (C) and podocyte number (WT-1–positive cells/glomeruli) (D) of control mice, STZ-D2, or STZ-D2 cotreated with BQ-123. E: PAS staining of kidneys from STZ-D2 (3 weeks diabetic; left) and STZ-D2 cotreated with BQ-123 (right). F: The sclerosis index score from STZ-D2 ± BQ-123 was performed as previously described (58), and glomeruli were graded as follows: 0 represents no lesion, 1+ represents sclerosis of <25% of the glomerulus, whereas 2+, 3+, and 4+ represent sclerosis of >25–50, >50–75, and >75% of the glomerulus, respectively. WT-1–positive cell counts/glomeruli per section in D are mean ± SEM of at least 180 glomeruli from 6 mice per group. *P < 0.05, ***P < 0.001 vs. control mice; +P < 0.05, ++P < 0.01 vs. diabetic mice.