Fig. 3.

Progressive neurodegeneration in sensory regions of dSap-r mutants. A. Representative images of 1 μm sections through 5-day-old and 22-day-old control (dSap-r^C27/+) and dSap-r mutant (dSap-r^C27/Df and dSap-r^C27/PBac) heads are shown at 20 × magnification (top 2 rows) and 63 × magnification (bottom 2 rows, eye (upper row) and antennal lobe (lower row)). Scale bars: 100 μm. B & C. Quantification of vacuole number was performed on 3 serial sections per fly (n ≥ 3 flies). Vacuoles were counted for the visual (B; eye, lamina, medulla and lobula complex) and olfactory (C; antennal lobes) systems at both sides of the brain. Quantification is shown for all controls and dSap-r mutants tested. Genotypes tested: +/+ (wild type); Df/+, PBac/+ and C27/+ (dSap-r heterozygotes); PBac/Df, C27/Df and C27/PBac (dSap-r mutants). Error bars: ± sem. ***p ≤ 0.001.