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. 2017 Feb;98:77–87. doi: 10.1016/j.nbd.2016.11.012

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Progressive lysosomal storage in dSap-r mutants. A. Western blots showing the abundance of the lysosomal proteins Arl-8 and cathepsin-L in 5-day-old and 22-day-old wild type (+/+), dSap-r^C27/Df (C27/Df), dSap-r^C27/PBac (C27/PBac) and dSap-r^PBac/Df (PBac/Df) mutants (n = 2). Tubulin abundance is shown as a loading control. Average band intensities and S.E.M are shown below. B. Confocal micrographs of 22-day-old wild type (+/+) and dSap-r^C27/Df mutant brains showing Arl-8 localisation and abundance (n > 8). Elav staining shows the similar orientation of both wild type and dSap-r^C27/Df mutant brains. Scale bar: 100 μm.