Fig. 5.

Neuronal swelling, degeneration and lipid storage are abundant in dSap-r mutant brains A. Transmission electron micrographs showing neuronal cell bodies adjacent to the antennal lobes of 22-day-old wild type (+/+) and dSap-r^C27/Df mutants (n = 3). Each soma has been demarcated in grey and rendered a different pseudocolour. The nuclei are demarcated by dashed lines. T, trachea; V, vacuole; white asterisk, fat body. Scale bar: 2 μm. B & C. Quantification of 22-day-old wild type (+/+) and dSap-r^C27/Df (C27/Df) mutant soma area (B) and cell:nucleus ratio (C). **p < 0.005. D. Transmission electron micrographs of 22-day-old wild type (+/+) and dSap-r^C27/Df mutant neuronal cell bodies surrounding the antennal lobes (n = 3). Multivesicular bodies and multilamellar bodies (arrows) are abundant in dSap-r^C27/Df mutant neuronal cell bodies. Scale bars: 2 μm (i–v), 100 nm (vi). E. Transmission electron micrographs showing the integrity of the ommatidia in 22-day-old wild type (+/+) and dSap-r^C27/Df mutants. Arrowheads mark vacuoles and arrows mark electron lucent material within regions of electron-dense storage. Scale bars: 5 μm (i & iv), 2 μm (ii & v) and 500 nm (iii & vi). F—I. Quantification of sphingosine levels (F), the sphingosine:ceramide ratio (G), the phosphatidylinositol level (H), and phosphatidylserine (I) in 5-day-old controls and dSap-r mutant brains. Genotypes tested: +/+ (wild type); Df/+, PBac/+ and C27/+ (dSap-r heterozygotes); PBac/Df, C27/Df and C27/PBac (dSap-r mutants). *p < 0.05, **p < 0.01, ***p < 0.000, ****p < 0.0001 as determined by ANOVA followed by post-hoc Bonferonni test.