Fig. 6.

Calcium homeostasis defects are associated with progressive deterioration of visual function in dSap-r mutants. A. 5-day-old (white bars) and 22-day-old flies (grey bars) were subjected to climbing assays (n > 20 flies). The climbing speed of dSap-r^C27 heterozygotes (C27/+), dSap-r^C27/Df (C27/Df) and dSap-r^C27/PBac (C27/PBac) mutants are shown. Error bars: ± sem. **p < 0.005 and ***p ≤ 0.001. B. Quantification of electroretinogram (ERG) amplitude and recovery rate after a blue light pulse. The recovery rate is the time taken for the potential to reach half way between the off-transient and base-line potentials after termination of the light pulse. C. Representative ERG traces for 5-day-old and 22-day-old control (dSap-r^C27/+) and dSap-r^C27/Df mutant females following a blue light pulse. D. Quantification of ERG amplitude after a blue light pulse in 5-day-old and 22-day-old wild type overexpressing the calcium exchanger CalX in the eye (CalX/+), dSap-r^C27/Df mutants (C27/Df) and dSap-r^C27/Df mutants overexpressing CalX in the eye (CalX/+;C27/Df). E. Representative ERG traces for 5-day-old and 22-day-old wild type overexpressing CalX (CalX/+), dSap-r^C27/Df mutants and dSap-r^C27/Df mutants expressing CalX (CalX; dSap-r^C27/Df). N ≥ 7 flies per condition. *** p ≤ 0.001.