Table 1. Theoretical amount of viremic blood, expressed as parts per blood meal (ppBM x 10−7) that would have to be introduced into the hemocoel per penetrating Brugia malayi microfilaria (MF) in order to result in the level of enhanced viral dissemination reported by Vaughan and Turell 1996 [8], Vaughan et al. 1999 [9].
| Mosquito species | Virus | Innate susceptibility (ID-50) * | Trial No. | Amount of virus ingested per mosquito (PFU) | Geometric mean number of MF penetrating the midgut | Net enhancement in viral dissemination | ppBM x 10−7 per MF |
|---|---|---|---|---|---|---|---|
| Ae. taeniorhynchus | VEEV | 0.20 | 1 | 250 | 17.5 (n = 4) | 61% (n = 13) | 587 |
| 2 | 160 | 13.7 (n = 5) | 50% (n = 8) | 899 | |||
| 3 | 100 | 12.6 (n = 6) | 62% (n = 60) | 2048 | |||
| 4 | 160 | 40.2 (n = 6) | 75% (n = 60) | 598 | |||
| Mean ± SD | 1033 ± 692 | ||||||
| EEEV | 0.09 | 1 | 630 | 27.3 (n = 5) | 54% (n = 31) | 56 | |
| 2 | 4000 | 3.3 (n = 5) | 16% (n = 23) | 33 | |||
| Mean ± SD | 44 ± 16 | ||||||
| Ae. aegypti | VEEV | 0.47 | 1 | 80 | 77.9 (n = 5) | 11% (n = 30) | 174 |
| EEEV | 0.10 | 1 | 400 | 111.9 (n = 5) | 10% (n = 32) | 9 |
*Number of plaque-forming units (PFU) of virus needed to infect 50% of the mosquitoes when injected into their thoraces.
Raw data and dose-response equations are presented in S1 Table.