Table 3. Detection of virus associated with Brugia malayi microfilariae (MF) incubated for 1 hour with either Venezuelan equine encephalitis (VEEV) or eastern equine encephalitis (EEEV) viruses and then extensively washed by repeated cycles of centrifugation, removal of supernatant and resuspension.
Viruses were detected in the final MF pellet by standard plaque assay and via inoculation into Aedes aegypti mosquitoes.
| Virus | --------- Virus titer (log10 PFU per ml) of supernatant at each dilution --------- | Virus titer of MF pellet (log10 PFU per ml) | Infection of mosquitoes inoculated with MF pellet | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Stock | 10−1 | 10−2 | 10−3 | 10−4 | 10−5 | 10−6 | 10−7 | 10−8 | |||
| EEEV + MF | 3.5 | 2.4 | 1.5 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 / 24 |
| VEEV + MF | 5.6 | 4.6 | 3.5 | 2.7 | 2.6 | 2.3 | 2.3 | 2.3 | 2.3 | 4.4 | 24 / 24 |