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. 2017 Feb 21;12(2):e0171930. doi: 10.1371/journal.pone.0171930

Fig 1. Flow cytometry strategy to determine mitochondrial and membrane damage on monocytes from PBMC.

Fig 1

Panel A shows the FSC-H x SSC-H plot and the gate to select the monocyte population in cultured PBMCs. Panel B shows the DIOC6 x EB plot in monocytes from non-stimulated cultures. Panel C shows the DIOC6 x EB plot in monocytes PPD-stimulated cultures. Insets shows in panel A the percentage of cells within the monocyte gate, and in panels B and the percentage of cell with mitochondrial damage (DIOC6 low), membrane damage (EB+), or live cells DIOC6 high).