Table 1. Primary antibodies used for immunodetection.
Name | Species | Manufacturer | Product number | Application | Dilution |
---|---|---|---|---|---|
pEGFR | Rabbit | Cell Signaling | 3777 | WB, IF | 1:1000(WB),1:100(IF) |
EGFR | Rabbit | Cell Signaling | 4267 | WB, IF | 1:1000(WB),1:100(IF) |
pP38MAPK | Rabbit | Cell Signaling | 4511 | WB, IF | 1:1000(WB),1:100(IF) |
P38MAPK | Rabbit | Cell Signaling | 9212 | WB | 1:1000 |
pJNK | Rabbit | Cell Signaling | 4668 | WB | 1:1000 |
pJNK | Mouse | Cell Signaling | 9255 | IF | 1:100 |
JNK | Rabbit | Cell Signaling | 9252 | WB | 1:1000 |
pERK | Rabbit | Cell Signaling | 4370 | WB, IF | 1:1000(WB),1:100(IF) |
ERK | Rabbit | Cell Signaling | 9102 | WB | 1:1000 |
p-c-Jun | Rabbit | Cell Signaling | 3270 | WB, IF | 1:1000(WB),1:100(IF) |
c-Jun | Rabbit | Cell Signaling | 9165 | WB | 1:1000 |
pMAPKAPK2 | Rabbit | Cell Signaling | 3007 | WB, IF | 1:1000(WB),1:100(IF) |
MAPKAPK2 | Rabbit | Cell Signaling | 3042 | WB | 1:1000 |
pHSP27 | Rabbit | Cell Signaling | 9709 | WB, IF | 1:1000(WB),1:100(IF) |
HSP27 | Mouse | Cell Signaling | 2402 | WB | 1:1000 |
BrdU | Mouse | Proteintech | 66241 | IF | 1:500 |
WB, Western blot; IF, immunofluorescence.