Table 3.

Inflammatory gene expression changes between infarcted (saline orMMP-9i groups) and no MI (D0 controls), as well as between MMP-9 inhibitor treated and vehicle control groups (MMP-9i vs saline). The increase, decrease, and no change were based on quantitative measurements that were statistically different based on RT²-PCR analysis for n = 6 LV infarct/group (see Supplemental Table 3 for quantitative data on all 84 genes). Qualitative directions of change for each group are shown here. Out of 84 genes measured, only these 40 genes at D1 and 38 genes at D7 were significantly different among groups (all p < 0.05).

	Saline vs Day 0	MMP–9i vs Day 0	MMP–9i vs Saline	Interpretation
Day 1 post–MI
(a): Cxcl5, Il11 (p): Ccl2, Ccl3, Ccl7, Ccl9, Ccr1, Cxcl1, Il1b, Il1r1, Il1r2, Il2rg, Il6st, Il8rb, Itgam, Spp1	↑	↑		↑ with MI
(a): Il10 (p): Ccl4, Ccr8, Il1f6, Lta	↑			MI effect blunted by MMP–9i
(a): Il3 (p): Xcr1		↑		MMP–9i effect
(a): Bcl6, Il10rb (p): Abcf1, C3, Ccl11, Cxcl9, Ccr10, Il15, Mif, Scye1, Tollip	↓	↓		↓ with MI
(p): Ccl25	↓			MI effect blunted by MMP–9i
(a): Tgfb1 (p): Cxcl10	↑		↓	MI effect blunted by MMP–9i
(p): Il16	↓	↓	↓	MI effect exacerbated by MMP–9i
(p): Cxcl11, Tnfrsf1a		↓		MMP–9i effect
Day 7 post–MI
(a): Il10ra, Ccl17 (p): Il1r1, Cx3cl1, Cxcr3	↑	↑		↑ with MI
(p): Casp1, Lta	↑			MI effect blunted by MMP–9i
(p): Il18, Il6ra	↑	↑	↑	MI effect exacerbated by MMP–9i
(a): Il11 (p): Ccl4, Ltb, Ccl8, Ccr5, Itgam, Itgb2, Spp1, Pf4		↑		MMP–9i effect
(a): Ccl6 (p): Ccl2			↑	MMP–9i effect
(a): Cxcl5 (p): Cxcr5, Ccl12,Ccl7, Ccl9, Ccr1, Ccr2, Ccr3, Tnfrsf1b		↑	↑	MMP–9i effect
(a): Il10rb, Tollip (p): Ccl11, Ccl24, Il15, Ccr9, Scye1	↓	↓		↓ with MI
(p): Il16	↓		↑	MI effect blunted by MMP–9i
(p): Abcf1	↓	↓	↓	MI effect exacerbated by MMP–9i

Arrows depict direction of change. (a) - anti-inflammatory; D - day; MI-myocardial infarction; (p) - pro-inflammatory. The bold black line separates the genes with increased expression from the genes that showed decreased expression at D1 and D7 post-MI. Gene names are provided in the Supplemental methods.