Table 1.
Genotypes of mutants from primary screen. Polymorphisms relative to M. smegmatis mc2155. Coordinates based on position in open reading frame except for B-P7, where genome position is used.
|
Mutant |
Allele |
Gene(s) Mutated |
Consequence |
|---|---|---|---|
A-A13 |
rplO-1 |
rplO 82ΔT (L15) |
L15 frameshift @ AA 28 of 147 |
A-A15 |
rplO-2 |
rplO 77ΔA (L15) MSMEG_3872 398ΔTC |
L15 frameshift @ AA 26 of 147 Precorrin-8X methylmutase frameshift AA 133 0f 208 |
A-G16 |
rrlB-2 |
rrlB G2296T |
non-coding, 23S rRNA |
A-G20 |
rrlB-4/lfrR-3 |
rrlB C904A lfrR T452C |
non-coding, 23S rRNA LfrR F151S |
A-H2 |
lfrR-1 |
lfrR C31A |
LfrR R11S |
A-I6 |
lfrR-4 |
lfrR 63+C |
LfrR frameshift @ AA 22 of 189 |
A-K20 |
rpsE-1 |
rpsE 599-606ΔAGAGTGAA |
S6 frameshift @ AA 200 OF 214 |
A-K24 |
rplO-3 |
rplO 74-77ΔGTGA |
L15 frameshift @ AA 26 of 147 |
A-L8 |
rrlB-1 |
rrlB G2199T |
non-coding, 23S rRNA |
A-M4 |
rplO-2 |
rplO 77ΔA |
L15 frameshift @ AA 26 of 147 |
A-M17 |
rplF-1 |
rplF 481-482ΔAA |
L6 frameshift @ AA 161 of 179 |
B-A9 |
lfrR-2 |
lfrR C98A |
LfrR A33E |
B-I24 |
rplO-3 |
rplO 74-77ΔGTGA |
L15 frameshift @ AA 26 of 147 |
B-O3 |
rplY-1 |
rplY 45ΔG |
L25 frameshift @ AA 16 of 215 |
B-O11 |
rpsE-2 |
rpsE 630+GC |
S6 frameshift @ AA 210 OF 214 |
B-O18 |
rrlB-3 |
rrlB G2891C |
non-coding, 23S rRNA |
B-P7 |
rplW/B-1 |
1539202ΔGCAGAGA* |
intergenic |
B-P17 |
rrlB-3 |
rrlB G2891C |
non-coding, 23S rRNA |