Table 2.

Evolved strains with additional mutations.

DOI: http://dx.doi.org/10.7554/eLife.20420.017

Name	Parent strain	Phenotype	Second mutation	Consequence
rplO-lfrR-1	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR 427+C	LfrR frameshift
rplO-lfrR-2	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR 440+C	LfrR frameshift
rplO-lfrR-3	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR T128G	LfrR V43G
rplO-lfrR-4	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR 172+ACC	LfrR 58+H
rplO-lfrR-5	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR 440+C	LfrR frameshift
rplO-lfrR-6	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	lfrR 118+G	LfrR frameshift
rplO-gyrA-1	rplO-1 (A-A13)	CIP MIC > 3.5 µg/ml	gyrA G265C	GyrA G89A
rplO-1r1	rplO-1 (A-A13)	wt growth rate	rplO 89+G	L16 SK28-29RR
rplO-1r2	rplO-1 (A-A13)	wt growth rate	rplO 61-96Δ*	L16 ΔRVGRGEGRRGKT
rplO-1r3	rplO-1 (A-A13)	wt growth rate	rplO 80+G	L16 S28P
rplO-gyrA-1r6	rplO-gyrA-1	wt growth rate	rplO 89+G	L16 SK28-29RR

*=CGCGTCGGTCGTGGTGAGGGCCGAAGGGCAAGACC.