Video 5. Mtb grows inside a dead macrophage treated with IFNγ prior to infection.
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DOI: 10.7554/eLife.22028.019
The left panel of the montage is a movie of macrophage infection at a resolution of 10 min between image acquisitions. The border of the analyzed macrophage is shown as a green outline, and the presence of DRAQ7 is shown in blue. RFP-expressing Mtb are in red. Time is hours:minutes. Scale bar is 20 µm. The graph on right shows the fluorescence signal converted to bacterial numbers in the analyzed macrophage. Timing of macrophage death was determined by the lack of internal movement and confirmed by a DRAQ7 entry (vertical line of x’s).