Table 1.
ELMO1 is expressed in human TGFβ-deficient invasive anorectal SCC. Human anorectal tumor biopsies from male and female patients, aged 32–70, were analyzed for phosphorylated (activated) SMAD2 (pSMAD2) and ELMO1 by immunohistochemistry (IHC) (see Figure 7 and Figure 7—figure supplement 1). Loss of pSMAD2 correlated with increased ELMO1 expression in 5 out of 6 SCC samples. Scoring: (++), strong positive staining; (+), positive staining; (-), negative staining. Abbreviations: SCC: squamous cell carcinoma; AIN3: Anal intraepithelial neoplasia (early stage tumor).
| Diagnosis | Sex | Age | pSMAD2 | ELMO1 | Pictures |
|---|---|---|---|---|---|
| normal anorectal mucosa | M | 58 | ++ | − | Figure 7A |
| SCC in situ | M | 47 | ++ | − | Figure 7—figure supplement 1A |
| SCC in situ + focus of invasion | M | 37 | ++ | − | |
| SCC in situ with microinvasion | M | 69 | ++ | − | |
| SCC in situ + invasive SCC | F | 70 | ++ and − | − | |
| AIN3 | F | 32 | ++ | − | Figure 7B |
| AIN3 | M | 52 | ++ | − | |
| invasive SCC + AIN3 | M | 61 | ++ and − | + | |
| invasive SCC + AIN3 | M | 52 | ++ and − | + | Figure 7—figure supplement 1B |
| invasive SCC grade 2 | M | 51 | ++ | − | |
| invasive SCC grade 2 | M | 59 | ++ | − | |
| invasive SCC grade 2 | F | 51 | ++ | − | |
| invasive SCC grade 3 | M | 53 | ++ | − | |
| invasive SCC grade 3 | F | 55 | ++ and − | + | Figure 7C |
| invasive SCC grade 3 | F | 53 | + and − | ++ | Figure 7D |
| invasive SCC grade 3 | M | 48 | − | ++ | Figure 7E |