Table 3.
Angiogenesis-related protein array. Decellularized adventitia from normal (n=7 patients pooled) and aneurysmal (n=28 pooled patients) human aorta, porcine adventitia and SIS were analyzed for 55 angiogenesis-related proteins. Values represent mean pixel density of two assay replicates ± standard deviation (S.D.) for chemiluminescence detected after 5 (human ECMs) or 14 (porcine ECMs) minutes of exposure.
| Porcine | Human | |||||
|---|---|---|---|---|---|---|
| Array Key | Protein | Gene ID |
Adventitia Mean (S.D.) |
SIS Mean (S.D.) |
Adventitia- Normal Aorta Mean (S.D.) |
Adventitia - Aneurysm al Aorta Mean (S.D.) |
| A1, A2 | Reference spots | N/A | - | - | - | - |
| A5, A6 | Activin A | 3624 | 44.9 (0.27) |
44.5 (0.08) |
25.6 (0.52) | 22.2 (0.14) |
| A7, A8 | ADAMTS-1 | 9510 | 43.7 (0.56) |
47.2 (2.41) |
20.6 (0.09) | 18.4 (0.33)# |
| A9, A10 | Angiogenin | 283 | 42.5 (0.09) |
51.4 (1.27) |
23.3 (0.07) | 18.5 (0.30)# |
| A11, A12 | Angiopoietin-1 | 284 | 43.6 (0.41) |
47.3 (1.51) |
21.2 (0.06) | 18.7 (0.30) |
| A13, A14 | Angiopoietin-2 | 258 | 45.5 (0.17) |
47.8 (0.03)* |
23.2 (0.05) | 20.1 (0.24)# |
| A15, A16 | Angiostatin/Plasminogen | 5340 | 42.9 (0.22) |
43.5 (0.61) |
19.8 (0.00) | 17.7 (0.32) |
| A17, A18 | Amphiregulin | 374 | 43.8 (0.32) |
43.7 (0.20) |
20.7 (0.09) | 18.0 (0.15)# |
| A19, A20 | Artemin | 9048 | 44.7 (0.08) |
43.6 (0.34) |
22.6 (0.35) | 19.9 (0.05)# |
| A23, A24 | Reference spots | N/A | - | - | - | - |
| B1, B2 | Coagulation factor III | 2152 | 44.3 (0.44) |
44.0 (0.71) |
20.2 (0.60) | 18.7 (0.09) |
| B3, B4 | CXCL16 | 58191 | 42.5 (0.24) |
43.6 (0.36) |
21.2 (0.49) | 21.4 (0.30) |
| B5, B6 | DPPIV | 1803 | 44.0 (0.05) |
41.3 (0.51) |
17.2 (0.05) | 16.7 (0.13) |
| B7, B8 | EGF | 1950 | 44.7 (0.08) |
44.9 (1.88) |
19.2 (0.23) | 18.3 (0.22)# |
| B9, B10 | EG-VEGF | 84432 | 46.3 (0.03) |
50.8 (1.58) |
20.2 (0.15) | 19.5 (0.20) |
| B11, B12 | Endoglin | 2022 | 44.0 (0.27) |
45.4 (1.47) |
20.6 (0.17) | 18.6 (0.20)# |
| B13, B14 | Endostatin/Col 18 | 80781 | 42.2 (0.11) |
43.9 (0.09)* |
20.0 (0.31) | 19.7 (0.04) |
| B15, B16 | Endothelin-1 | 1906 | 44.9 (0.40) |
45.6 (0.67) |
22.2 (0.48) | 21.5 (0.22) |
| B17, B18 | FGF-acidic | 2246 | 43.3 (0.70) |
122.0 (4.43)* |
21.7 (0.05) | 19.5 (0.18)# |
| B19, B20 | FGF-basic | 2263 | 43.5 (0.46) |
100.2 (0.56)* |
22.9 (0.00) | 20.6 (0.01)# |
| B21, B22 | FGF4 | 2249 | 44.6 (0.18) |
43.4 (0.74) |
20.3 (0.26) | 18.2 (0.37)# |
| B23, B24 | FGF7 | 2252 | 43.9 (0.38) |
45.0 (0.70) |
19.8 (0.22) | 19.7 (0.00) |
| C1, C2 | GDNF | 2668 | 43.7 (0.74) |
44.7 (0.79) |
20.0 (0.13) | 18.0 (0.58) |
| C3, C4 | GM-CSF | 1437 | 44.4 (0.16) |
43.9 (0.31) |
22.9 (0.34) | 22.2 (0.49) |
| C5. C6 | HB-EGF | 1839 | 45.1 (0.16) |
43.7 (0.06)* |
20.4 (0.19) | 19.7 (0.19) |
| C7, C8 | HGF | 3082 | 44.6 (0.84) |
46.0 (1.17) |
22.1 (0.03) | 20.6 (0.51) |
| C9, C10 | IGFBP-1 | 3484 | 44.9 (0.10) |
50.1 (0.53)* |
22.0 (0.04) | 21.1 (0.06)# |
| C11, C12 | IGFBP-2 | 3485 | 42.2 (0.25) |
44.6 (1.19) |
19.5 (0.10) | 18.3 (0.05)# |
| C13, C14 | IGFBP-3 | 3486 | 45.5 (0.37) |
45.7 (0.16) |
22.1 (0.22) | 21.3 (0.13) |
| C15, C16 | IL1-β | 3553 | 42.8 (0.22) |
43.1 (0.16) |
19.2 (0.21) | 18.7 (0.23) |
| C17, C18 | IL-8 | 3576 | 42.0 (0.05) |
45.7 (0.50) |
19.4 (0.02) | 18.5 (0.17) |
| C19, C20 | LAP (TGF-β1) | 7040 | 41.5 (1.52) |
46.0 (0.61) |
22.1 (0.08) | 20.0 (0.19)# |
| C21, C22 | Leptin | 3952 | 43.3 (0.09) |
43.6 (0.91) |
21.8 (0.12) | 19.8 (0.04)# |
| C23, C24 | MCP-1 | 6347 | 43.1 (0.79) |
44.6 (0.28) |
23.2 (0.22) | 20.8 (0.61) |
| D1, D2 | MIP-1α | 6348 | 43.8 (0.13) |
44.8 (0.52) |
22.4 (0.12) | 20.9 (0.01)# |
| D3, D4 | MMP-8 | 4317 | 43.6 (0.31) |
43.3 (0.15) |
21.1 (0.85) | 20.8 (0.47) |
| D5, D6 | MMP-9 | 4218 | 44.9 (0.04) |
44.4 (0.39) |
21.1 (0.09) | 21.0 (0.02) |
| D7, D8 | NRG1-β1 | 3084 | 43.2 (0.28 ) |
44.3 (1.45) |
20.9 (0.02) | 20.3 (0.33) |
| D9, D10 | Pentraxin 3 | 5806 | 44.5 (0.36) |
48.8 (0.07)* |
21.7 (0.16) | 21.3 (0.18) |
| D11, D12 | PD-ECGF | 1890 | 46.3 (0.23) |
46.8 (1.67) |
21.7 (0.51) | 21.3 (0.38) |
| D13, D14 | PDGF-AA | 5154 | 44.7 (0.12) |
44.4 (0.05) |
21.6 (0.04) | 21.0 (0.15) |
| D15, D16 | PDGF-BB | 5155 | 41.6 (0.19) |
42.3 (0.32) |
18.5 (0.41) | 18.5 (0.26) |
| D17, D18 | Persephin | 5623 | 45.0 (0.11) |
44.7 (0.63) |
22.9 (0.24) | 21.4 (0.10)# |
| D19, D20 | Platelet factor 4 | 5196 | 41.8 (0.66) |
43.2 (0.07) |
21.9 (0.13) | 19.5 (0.43) |
| D21, D22 | PlGF | 5228 | 47.0 (0.47) |
48.5 (0.25) |
25.5 (0.32) | 22.9 (0.14)# |
| D23, D24 | Prolactin | 5617 | 42.6 (0.34) |
44.2 (0.81)* |
21.6 (0.50) | 19.5 (0.16) |
| E1, E2 | Serpin B5 | 5268 | 41.8 (0.51) |
48.4 (0.44)* |
23.2 (0.25) | 21.5 (0.17)# |
| E3, E4 | Serpin E1 | 5054 | 42.4 (0.69) |
43.2 (0.47) |
22.5 (0.46) | 20.6 (0.49) |
| E5, E6 | Serpin F1 | 5176 | 43.2 (0.21) |
44.0 (0.18) |
21.6 (0.15) | 22.2 (1.00) |
| E7, E8 | TIMP-1 | 7076 | 43.7 (0.19) |
44.6 (1.09) |
20.3 (0.25) | 19.5 (0.18) |
| E9, E10 | TIMP-4 | 7079 | 43.8 (0.68) |
47.5 (0.42)* |
21.0 (0.32) | 19.9 (0.52) |
| E11, E12 | Thrombospondin-1 | 7057 | 86.2 (0.22) |
88.9 (3.50) |
63.3 (1.42) | 60.0 (1.91) |
| E13, E14 | Thrombospondin-2 | 7058 | 43.5 (1.09) |
44.0 (0.08) |
21.1 (0.05) | 20.8 (0.07) |
| E15, E16 | uPA | 5328 | 43.1 (0.77) |
45.2 (0.15) |
20.5 (0.45) | 20.5 (0.32) |
| E17, E18 | Vasohibin | 22846 | 44.7 (0.27) |
44.1 (0.30) |
21.2 (0.39) | 20.3 (0.16) |
| E19, E20 | VEGF | 7422 | 44.1 (0.44) |
44.2 (0.38) |
22.0 (0.29) | 19.9 (0.33)# |
| E21, E22 | VEGF-C | 7424 | 45.5 (0.03) |
44.4 (0.49) |
21.0 (0.00) | 18.7 (0.31) |
| F1, F2 | Reference spots | N/A | - | - | - | - |
| F23, F24 | Negative control spots | N/A | - | - | - | - |
*
p<0.05 when compared with porcine adventitia;
#
p<0.05 when compared with normal human aortic adventitial specimens.