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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Bioorg Med Chem. 2016 Nov 10;25(3):813–824. doi: 10.1016/j.bmc.2016.11.013

Figure 5.

Figure 5

Effects of RpMetAP inhibitors on R. prowazekii growth. The loss of infected host ECs viability due to normal R. prowazekii growth was used to indirectly assess whether RpMetAP inhibitors produced antibacterial effects. The expectation here is that compounds inhibiting rickettsial growth will restore host EC viability to levels comparable to the uninfected control ECs. Host EC viability was measured via WST-1 metabolism (calculated as A450nm – A660nm and normalized to media only as background). Solid black bars denote compounds that restored host EC viability to levels comparable to uninfected ECs (denoted by the dashed lines). U = uninfected control ECs; Rp = R. prowazekii-infected host ECs; Rp+DM = R. prowazekii-infected host ECs treated with the vehicle/solvent control (0.3% DMSO); numbers denote compound identification from Table 6. Infections were performed at a MOI = 100 rickettsiae: 1 host cell. Results represent the average of triplicate infections ± standard deviation.