(A and B) Genomic regions of the Lhx8 (A) and Gli2 (B) loci with the ChIP-seq datasets and genomic features shown; NKX2-1 ChIP-seq, H3K4me3, H3K4me1, H3K27ac, H3K27me3, UCSC genes, and mammalian conservation. The H3 modification ChIP-seqs were performed on both WT and Nkx2-1cKO MGE at E13.5. Called TF binding peaks are labeled with an asterisk.
(C–F) In situ analysis of Lhx8 (C and D) and Gli2 (E and F) in WT and Nkx2-1cKO forebrain at E13.5.