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. Author manuscript; available in PMC: 2017 Feb 21.
Published in final edited form as: Nature. 2016 Jun 15;534(7608):562–565. doi: 10.1038/nature18595

Extended Data Figure 7. SAXS reconstruction of Izumo1 and Juno mutants.

Extended Data Figure 7

(a) Ab initio small-angle X-ray scattering (SAXS) reconstruction, experimental scattering curves, normalized pair distance distribution function, P(r) and Kratky plot showing the degree of flexibility of (a) WT Izumo122-254-E45K Juno20-228, (b) WT Izumo122-254-K163E Juno20-228, (c) E71K Izumo122-254-WT Juno20-228, and (d) R160E Izumo122-254-WT Juno20-228 complexes. No concentration-dependent or radiation effects were observed. The inset box shows linearity in the Guinier plot at low q (qRg <1.3). The WT Izumo122-254-WT Juno20-228 complex crystal structure was docked into the SAXS reconstructed molecular envelopes. (e) Summary of the experimentally derived SAXS parameters for the various Izumo1-Juno complexes. The program SCATTER47 was used to calculate the radius of gyration (Rg), maximum linear dimension (Dmax), and to perform Porod-Debye analysis to obtain the Porod volume and P coefficient.