Skip to main content
. Author manuscript; available in PMC: 2017 Feb 21.
Published in final edited form as: Methods Cell Biol. 2016 Apr 4;134:335–368. doi: 10.1016/bs.mcb.2016.03.002

Figure 10.

Figure 10

Optical mapping of cardiac conduction reveals aberrant calcium excitation in tre mutants. Sequence of heat map images depicts calcium activation in a wild-type (WT) heart expressing Tg(myl7:gCaMP) at 36 hpf; isochronal lines represent every 33 milliseconds. In WT embryos, calcium activation initiates in the sinus venosus (bottom) and concludes in the ventricle (top). In contrast, heat map images from a tre mutant heart indicate that only sporadic calcium activation occurs in the absence of Ncx1h function. Adapted from Shimizu et al. (2015).