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ETD spectrum of peptide 143-YALFYK-148 and its oxidation products. (A) Unoxidized peptide 173-YALFYK-178. (B) Mixture of singly oxidized isomers. By measuring the ratio of oxidized product ions to total product ions for each fragment, oxidation can be quantified to occur on F176 and the Y173-A174 fragment (on the basis of CID data and the relative reactivities of alanine and tyrosine, almost no oxidation occurs on A174).
