Figure 3.
Activation of Ca2+ responses by streptavidin (SA) crosslinking of biotinylated huIgE bound to the chimeric IgE receptor αζζ expressed in RBL-2H3 mast cells is enhanced ~30–50% by cytochalasin D (cyto D) added after (A) or before (B) 3 nM SA. Ca2+ responses were monitored by steady-state fluorimetry at 37°C in a basal salt solution. Each addition of cyto D is 1 μM.