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. 2017 Feb 21;7:45. doi: 10.1186/s13568-017-0342-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Complementation of Saccharomyces cerevisiae tkl1 mutant by MmTKL1 and MmTKL2. This assay tested complementation of the aromatic amino acid (AAA) auxotrophy of the S. cerevisiae BY4741 Δtkl1 mutant by plasmids harboring MmTKL1, MmTKL2, or ScTKL1 (the endogenous S. cerevisiae gene) or with no insert (pDB05). Also tested were the untransformed mutant and the wild-type parent (BY4741). Growth was tested by spotting serial dilutions at an estimated 10⁵–10⁰ cells/spot, left to right on parallel synthetic complete (SC) agar medium with or without added AAAs (tryptophan, tyrosine, phenylalanine) and incubating the plates at 30 °C for 3 days