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. 2017 Feb 21;7:45. doi: 10.1186/s13568-017-0342-0

Fig. 7.

Fig. 7

Gene expression and intracellular erythritol accumulation during long-term growth. M. megachiliensis was cultivated at 30 °C for 72 h in GY medium containing 20% glucose. Every 12 h, samples were removed for analysis. Gene expression was analyzed by real-time PCR, and normalized using GAPDH expression level. Intracellular erythritol content was determined chromatographically with an ICS-3000 system, and is presented as µg/mg dry cells