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. 2016 Nov 29;101(6):2319–2331. doi: 10.1007/s00253-016-7993-7

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Conversions of a omeprazole and b rosiglitazone by P450_BM3 and PTDH-P450_BM3. Substrate was used at concentrations of 10 mM in a total volume of 100 mL. Reactions were carried out at 30 °C; reaction progress was measured after 4 h in comparison to 0 h samples by UPLC-MS. Phosphite-driven reactions contained phosphite and NADP⁺, whereas NADPH-driven reactions contained NADPH and phosphite. The yield of product and remaining substrate was determined as a percentage of the respective peak area to the total related peak area. Reactions were performed in duplicate (5 = omeprazole, 6 = monohydroxylated omeprazole, 7 = rosiglitazone, 8 = demethylated rosiglitazone, 9 = monohydroxylated rosiglitazone)