Table 1.

Kinetics of NADPH dependent lauric acid oxidation. Kinetic parameters of PTDH-P450_BM3 and P450_BM3 for NADPH and lauric acid were analyzed by following absorption of NADPH in a spectrophotometer at 340 nm. Specific activities were determined with 100 nM of the respective enzyme and varying concentrations of either NADPH or lauric acid. When fixed, NADPH had a concentration of 200 μM and lauric acid of 2.5 mM

	NADPH			Lauric acid
	k cat [s−1]	K m [μM]	k cat/K m [s−1 mM−1]	k cat [s−1]	K m [μM]	k cat/K m [s−1 mM−1]
PTDH-P450BM3	6.9 ± 0.5	15.4 ± 2.3	448	8.0 ± 0.3	688 ± 78	11.6
P450BM3	6.0 ± 0.3	17.8 ± 2.7	337	7.3 ± 0.2	1427 ± 113	5.1