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. 2004 Dec;15(12):5255–5267. doi: 10.1091/mbc.E04-06-0443

Figure 2.

Figure 2.

Spc7 localizes to the kinetochore. (A) Localization of the Spc7-GFP protein in wild-type cells in interphase (a) early (b) or late (c) stages of mitosis. Fixed cells were stained with DAPI and anti-GFP antibody. (B) Localization of the Spc7 fusion in a wild-type cell arrested by overexpression of mph1+. GFP signals; spindles and condensed chromosomes were simultaneously observed by staining with anti-GFP antibody, anti-tubulin antibody and DAPI. (C) Colocalization of Spc7-HA (green) and Mal2-GFP (red) fusion proteins in interphase cells (e) and cells arrested by overexpression of mph1+ (f). Chromosomes, HA- and GFP-signals were simultaneously observed by staining with anti-GFP antibody, anti-HA antibody, and DAPI. Bar, 10 μm.