Table 3.

ErbB2 redistribution on MVB-limiting membranes after 45 min with GA

	ErbB2 endocytic (% of NB)	ErbB2 endo/lyso coated limiting mb (% of limiting mb)	TfR endo/lyso coated limiting mb (% of limiting mb)
Control	13.6 ± 1.2	11.9 ± 3.8	8.3 ± 0.6
GA	22.1 ± 3.9	38.9 ± 3.5a	10.9 ± 2.7

SKBr3 cells were incubated ± GA for 45 min at 37°C and then fixed and processed for quantitative detection of ErbB2 or TfR by immunogold electron microscopy as described in Materials and Methods. Normalization to the total number of gold particles on the endosomal limiting membrane was performed per sample of 25 endosomal vacuoles prior to mean and SE calculations (n = 3). NB, non-biosynthetic; endo/lyso, endosomes and lysosomes; mb, membrane.

^aStatistical significance relative to control (p < 0.01).