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. 2004 Dec;15(12):5318–5328. doi: 10.1091/mbc.E04-05-0385

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Copyright © 2004, The American Society for Cell Biology

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Figure 6. — Localization of GFP-TPX2 fragments on spindles and asters assembled in Xenopus egg extracts. (A) GFP-TPX2 fragments (0.1 μM) were added to egg extracts as they were cycled back into mitosis. Their localization was examined on spindles assembled around sperm nuclei. In the overlays, microtubules are in red, GFP-fragments in green, and chromatin in blue. Bar, 10 μm. (B) TPX (241-715) and TPX (319-715) (0.1 μM) were added to a TPX2-depleted extract. Microtubule asters formation was induced by addition of purified centrosomes. In the overlays, microtubules are in red, and GFP-fragments in green.